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免疫塞内卡病毒灭活疫苗后豚鼠、家兔与猪的血清中和抗体相关性研究
齐志涛,方建国,王娥娥,郝苏云,高艳华,路荣,赵丽霞,范秀丽,张燕红,杜宇荣,陈九连,宋庆庆
0
(金宇保灵生物药品有限公司)
摘要:
为研究免疫塞内卡病毒(SVA)灭活疫苗后豚鼠、家兔与猪血清中和抗体的相关性,分别用0.25、0.5、1.0、2.0 mL的SVA灭活疫苗接种豚鼠和家兔,同时以2.0 mL的SVA灭活疫苗接种猪,分别于免疫前以及免疫后第7天、第14天、第21天、第28天采血液,测定各动物血清中SVA中和抗体,利用Microsoft Excel软件对所得结果进行整理并分析豚鼠与猪血清SVA中和抗体、家兔与猪血清SVA中和抗体之间的线性关系,进一步采用IBM SPSS Statistics 19软件对线性关系的显著性进行分析。结果显示,豚鼠和家兔均可产生SVA中和抗体,且免疫剂量越大,免疫动物产生的SVA中和抗体滴度越高。猪免疫后产生了SVA中和抗体,监测期内随着时间的延长,SVA中和抗体水平逐渐升高。当家兔和豚鼠免疫剂量为1.0 mL时,二者产生的SVA中和抗体与猪SVA中和抗体呈正相关,相关性分别为0.990和0.998,相关性极显著。研究表明,采用1.0 mL的剂量免疫豚鼠或家兔,能间接反应SVA灭活疫苗在猪体的SVA中和抗体水平。
关键词:  塞内卡病毒  灭活疫苗  效力检验
DOI:
投稿时间:2021-01-28修订日期:2021-07-01
基金项目:
Study on the Correlation of Antibody between Guinea Pigs and Pigs, Rabbits and Pigs after Immunized with SVA Inactivated Vaccine
(Jinyubaoling bio-pharmaceutical Co. , Ltd.)
Abstract:
To explore alternative methods for testing the efficacy of inactivated SVA vaccine, guinea pigs and rabbits were immunized with inactivated SVA vaccine at doses of 0.25 mL, 0.5 mL, 1.0 mL and 2.0 mL. At the same time,pigs were immunized with a dose of 2.0 mL. The serum neutralizing antibody against SVA was determined before vaccination and after vaccination on the 7th, 14th, 21 st and 28th. The results were obtained and preliminary analysis of the linear relationship between guinea pigs, rabbits and pigs SVA serum neutralizing antibodies were made by Microsoft Excel software, further more,the significance of the linear relationship were analyzed by IBM SPSS Statistics 19 software. As the result,SVA neutralizing antibody could be produced by guinea pigs and rabbits after immunized inactivated SVA vaccine, and the higher the immunization dose, the higher the neutralizing antibody titer produce. SVA neutralizing antibodies were produced by pig after immunized inactivated SVA vaccine, and with time, the level of SVA neutralizing antibodies gradually increases. When the dose was 1.0 mL, the correlation of SVA neutralizing antibody between guinea pigs and pig was 0.990,between rabbits and pig was 0.998, the correlation was extremely significant. It was concluded that,the efficacy of the inactivated SVA vaccine on pigs could be indirectly reflected by immunizing guinea pigs or rabbits with 1.0 mL.
Key words:  Senecavirus  inactivated vaccine  efficacy test  alternative method

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