| 摘要: |
| 为了改进猪圆环病毒2型的培养工艺,驯化了一株可无血清培养的全悬浮PK15细胞用于培养猪圆环病毒2型,并对病毒的敏感性、接毒时间、MOI、收获方法进行了试验。结果表明,该细胞培养猪圆环病毒2型,批次收获时,接毒时细胞密度为0.5×106/mL,接毒量为0.1 MOI,接毒后72 h后收毒,病毒滴度能达到106.4 TCID50/mL；如果采用连续收获,接毒和带毒传代时均使细胞密度为0.5×106/mL,接毒量为0.04 MOI,收毒时间为接毒后72 h,连续收获3代,病毒滴度分别为106 TCID50/mL、106.25 TCID50/mL、106.5 TCID50/mL,病毒滴度和收获液体积较为理想,以期为大规模培养猪圆环病毒2型提供参考。 |
| 关键词: 猪圆环病毒2型 全悬浮 PK15细胞 无血清 连续收获 |
| DOI： |
| 投稿时间：2021-08-31修订日期：2022-01-04 |
| 基金项目: |
|
| The study of suspended PK15 cells culture porcine circovirus type 2 by serum-free medium |
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| (Beijing Minhai Biological Technology Co.,Ltd) |
| Abstract: |
| In order to improve the culture of pocine circovirus type 2, a suspension PK15 cell line that could be cultured by serum-free medium was domesticated to culture pocine circovirus type 2 . The virus sensitivity, the infection time, MOI and harvest methods were investigated. Results shown that after 72 h of infection when using 0.1 MOI of pocine circovirus type 2 to infect the domesticated suspension PK15 cell in the cell density of 0.5×106 cells per 1 mL, the title of virus could reach to 106.4 TCID50/mL. In addition, when using 0.04 MOI of pocine circovirus type 2 to infect the domesticated suspension PK15, and continuous harvested three times after 72 h of infection, the virus title could reach to 106 TCID50/mL, 106.25 TCID50/mL, 106.4 TCID50/mL. In conlusion, this study provides a reference for large-scale culture of porcine circovirus type 2. |
| Key words: porcine circovirus type 2 suspension PK15 cells serum free continuous harvest |
