| 摘要: |
| 为了优化猪源嗜酸乳杆菌La-0231菌粉生产工艺。采用单因素试验、正交试验，优化发酵温度、培养基初始pH值、氢氧化钠流加浓度、发酵时间、离心时的流量设定值、预冻温度、冷冻干燥中物料仓的真空度，并对冻干菌粉进行了耐酸、耐胆盐性能测试。结果表明，优化的5吨生产罐发酵控制参数为：发酵温度37℃，培养基初始pH值为6.40，氢氧化钠的流加浓度为25％，发酵时间为16h，发酵液最终pH值范围在4.20~4.80之间，OD600nm值在6.00以上，发酵液的活菌数为6.80×109CFU/mL。离心时的流量设定值以216L/h为优。确定预冻温度为-45℃~-50℃最佳，冷冻干燥中物料仓的真空度设定以50Pa较为适宜。优化后猪源嗜酸乳杆菌La-0231菌粉生产工艺，冻干菌粉活菌数含量为4.56×1011CFU/g，水分含量3.19%；经耐酸、耐胆盐性能测试，显示其具有良好的耐酸、耐胆盐特性。是一株有待开发兽药前景的益生菌。 |
| 关键词: 猪源嗜酸乳杆菌 生产工艺优化 冻干菌粉 耐酸、耐胆盐特性 |
| DOI： |
| 投稿时间：2021-11-08修订日期：2022-05-04 |
| 基金项目: |
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| Optimization of Production Process of Lactobacillus acidophilus La-0231 Powder from Piglets |
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| (Shanghai Bornsun Bioengineering Co., Ltd.) |
| Abstract: |
| In order to optimize the production process of Lactobacillus acidophilus La-0231 powder from piglets, we used single factor experiments and orthogonal experiment to optimize the fermentation temperature, initial pH of medium, sodium hydroxide concentration, fermentation time, flow rate setting for centrifugation, prefreezing temperature, vacuum degree of material bin in freeze-drying and also tested the acid resistance and bile salt resistance of freeze-dried powder.The test results showed that the optimized fermentation control parameters of 5-ton fermenter are fermentation temperature 37℃, initial pH of medium 6.40, sodium hydroxide concentration 25%, fermentation time 16h, final pH value of fermentation broth 4.20~4.80, OD600nm value of fermentation broth above 6.00,?numbers of viable bacteria 6.80×109 CFU/mL.The flow rate setting value of 216L/h is the best when centrifuging.The optimum prefreezing temperature is-45℃~-50℃, the vacuum degree setting of material bin in
freeze-drying is 50Pa. After optimization, the viable counts of freeze-dried powder of Lactobacillus acidophilus La-0231 from piglets are 4.56×1011CFU/g and its moisture content is 3.19%?.The results of acid and bile salt resistance test showed that it has good acid and bile salt resistance. It is a probiotic to be exploited in veterinary medicine. |
| Key words: Lactobacillus acidophilus of piglets origin process optimization freeze-dried powder acid and bile salt resistance |
