| 摘要: |
| [摘 要] 建立了同时测定双葛止泻口服液中绿原酸和葛根素含量的测定方法。采用高效液相色谱（HPLC）法，使用Waters XBridge C18（250 × 4.6 mm，5 μm）色谱柱，流动相为乙腈-0.4%磷酸溶液梯度洗脱；检测波长为315 nm；流速为1.0 mL/min；柱温为30 ℃。结果表明，绿原酸浓度在0.008242~0.1030 mg/mL的范围内，与峰面积线性关系良好（r = 1.0000），平均回收率为99.1%（n = 6），RSD为1.8%；葛根素浓度在0.02296~0.2871 mg/mL的范围内，与峰面积线性关系良好（r = 1.0000），平均回收率为98.7%（n = 6），RSD为1.6%。该方法简单、准确、专属性强，可用于双葛止泻口服液中绿原酸和葛根素的质量控制。 |
| 关键词: 绿原酸 葛根素 双葛止泻口服液 高效液相色谱法 |
| DOI： |
| 投稿时间：2021-12-13修订日期：2022-09-09 |
| 基金项目: |
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| Determination of Chlorogenic Acid and Puerarin in Shuangge Zhixie Oral Liquid by HPLC |
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| (Luoyang Huizhong Veterinary Medicine Co. , Ltd) |
| Abstract: |
| Abstract: The method for determination of the content of chlorogenic acid and puerarin in Shuangge Zhixie Oral Liquid was developed. The analysis was performed on an Waters XBridge C18 column(250 × 4.6 mm, 5 μm). The gradient mobile phase was consisted of acetonitrile-0.4% phosphoric acid solution, and the detection wavelength was 315 nm. The flow rate was 1.0 mL/min and the column temperature was 30 ℃. The linear range of the calibration curves for chlorogenic acid was 0.008242~0.1030 mg/mL (r = 1.0000), and the average recovery was 99.1% (n = 6) with RSD of 1.8%. The linear range of the calibration curves for puerarin was 0.02296~0.2871 mg/mL (r = 1.0000), and the average recovery was 98.7% (n = 6) with RSD of 1.6%. The method is simple and accurate with high specificity, which can be used to determine the content of chlorogenic acid and puerarin in Shuangge Zhixie Oral Liquid. |
| Key words: chlorogenic acid puerarin Shuangge Zhixie oral liquid HPLC |
