| 摘要: |
| 建立高效液相色谱荧光检测法测定牛羊组织中碘醚柳胺残留量。牛羊组织中残留的碘醚柳胺，经乙腈-丙酮溶液提取，阴离子交换固相萃取柱净化，高效液相色谱-荧光法测定，外标法定量。碘醚柳胺在10～5000 ng/mL的浓度范围内呈现良好的线性关系，碘醚柳胺在牛羊肌肉、肝脏、肾脏、脂肪中的检测限均为2.5 μg/kg，定量限均为5 μg/kg，牛羊肌肉、肝脏、肾脏、脂肪中15～500 ng/g添加浓度范围内的回收率为77.4%～93.8%，批内批间RSD值均<15％。 |
| 关键词: 牛羊组织 碘醚柳胺残留 高效液相色谱法 |
| DOI： |
| 投稿时间：2022-01-26修订日期：2022-07-22 |
| 基金项目: |
|
| Determination of Rafoxanide Residues in the Tissue of Cattle and Sheep by Liquid Chromatography Method |
|
| (Animal feed quality and safety of venterinary testing centre in Liaoning province Shenyang China) |
| Abstract: |
| A method of high performance liquid chromatography for determination of rafoxanide in the tissue of cattle and sheep was developed. The samples were extracted with acetonitrile-acetone(80+20), and cleaned up with MAX solid phase extraction cartridg.The separation of the target was carried out on a C18column by elution with acetonitrile-0.1% formic acid(85∶15) as mobile phase, The results showed that correlation coefficient was 0.9999.The LOD and LOQ were 2.5 μg·kg-1 and 5.0 μg·kg-1.The average recoveries were between 77.4% and 93.8% at spiked levels of 15~500 μg·kg-1.The RSD were below 15%.The real sample tests showed this method can be used for the sensitive and accurate determination of rafoxanide residues in the tissue of cattle and sheep. |
| Key words: the tissue of cattle and sheep rafoxanide residues HPLC |
