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高效液相-串联质谱法测定猪、牛和羊尿液中18种同化激素的含量
侯轩,罗成江,王彬,黄娟,吴望君,陈晓林,任玉琴,陈洁
0
(浙江省动物疫病预防控制中心)
摘要:
建立了高效液相色谱-串联质谱(HPLC-MS/MS)检测动物尿液18种同化激素的方法。动物尿液在37 ℃(±0.5℃)下酶解16 h后,调节pH=7.0(±0.5℃),C18固相萃取柱净化,用HPLC-MS/MS进行检测。18种同化激素在1~ 500 μg/L浓度范围内线性关系良好(r2≥0.99),回收率在70.9 ~112%之间,日内变异系数范围为1.2 ~ 13.1%,日间变异系数范围为3.5~16.7%,检测限为0.5 μg/kg,定量限为1.0 μg/kg。本方法操作简便,灵敏度高,适用于动物尿液中18种同化激素的同时测定。
关键词:  同化激素  动物尿液  高效液相色谱-串联质谱法
DOI:
投稿时间:2022-03-31修订日期:2022-08-22
基金项目:浙江省农业重大技术协同推广计划(2021XTTGXM03-4)、浙江省重点研发计划项目(2020C02032)
Determination of 18 anabolic hormones in animal urine by HPLC-MS/MS
(Zhejiang Animal Disease Control and Prevention Center)
Abstract:
A method for the determination of 18 anabolic hormones in animal urine by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) was established. When it was enzymolysis for 16 h at 37℃ (±0.5℃), adjusted the pH to 7.0 (±0.5), the animal urine was purified by solid phase extraction column and detected by HPLC-MS/MS. The linear relationship of 18 assimilation hormones was good in the concentration range of 1 ~ 500 ng / mL(r2≥0.99), the recovery was 70.9 ~ 112%, the intra day coefficient of variation was 1.2 ~ 13.1%, the intra day coefficient of variation was 3.5 ~ 16.7%, and the detection limit was 0.5 μg/kg, limit of quantitation was 1.0 μg/kg.The method is simple, sensitive and suitable for the simultaneous determination of 18 anabolic hormones in animal urine.
Key words:  anabolic hormone  Animal urine  HPLC-MS/MS

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