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| 鸡毒支原体灭活疫苗效力检验气囊注射攻毒方法的研究 |
| 魏津,刘博,马欣,王甲,赵浩然,刘元杰,冯妍,刘业兵,罗玉峰 |
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| (中国兽医药品监察所) |
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| 摘要: |
| 鸡毒支原体灭活疫苗效力检验采用的喷雾攻毒方法所需菌液量大,结果易受喷雾时间和器械设备影响。为了探索一种简便、易行且结果稳定的新方法,本研究冻干了一批攻毒用菌株鸡毒支原体R株,对其性状、纯粹、真空度、剩余水分、培养特性、特异性、活菌计数和均一性等进行检定,结果全部符合规定。将其稀释成不同活菌数,采用气囊注射方式攻毒,观察试验结果并与《中国兽药典(三部)》中的喷雾方法进行比较。结果表明,建立的气囊注射攻毒法稳定、准确,通过5批次疫苗效力检验试验比较,与喷雾方法结果无显著差异,可为该类疫苗攻毒方法优化提供新思路。 |
| 关键词: 鸡毒支原体 灭活疫苗 气囊注射 效力检验 |
| DOI： |
| 投稿时间：2022-04-19修订日期：2022-10-08 |
| 基金项目: |
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| Research on Air Sacs Challenge Method of Potency Test of Mycoplasma gallisepticum Inactivated Vaccine |
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| (China Institute of Veterinary Drug Control) |
| Abstract: |
| The aerosol challenge method for potency test of Mycoplasma gallisepticum inactivated vaccine requires a large amount of live broth culture and the result is influenced by spraying time and equipment. In order to establish an new method with stable test results, one batch of freeze-drying Mycoplasma gallisepticum R strain culture was prepared in this study, and its characteristics, purity, vacuum degree, residual moisture content, specificity, viable count and homogeneity were determined, the results are confirmed to the regulations. Diluted the freeze-drying culture to different titration and injected into air sacs, observe the test results. Comparison with the aerosol challenge regulated in Veterinary Pharmacopoeia of People’s Republic of China show the method is stable and accurate. Five batches of vaccines potency tests show that there was no significant difference in results with aerosol challenge method, and can provide new ideas for the optimization of the challenge method of Mycoplasma gallisepticum vaccines. |
| Key words: mycoplasma galliscepticum inactivated vaccine injection into air sacs potency test |
