| 摘要: |
| 建立了硫酸新霉素可溶性粉中非法添加7种抗寄生虫药物的HPLC-PDA检测方法。采用十八烷基键合硅胶为填充剂；以水-乙腈为流动相，梯度洗脱，流速1.0 mL/min；二极管阵列检测器，波长为245 nm。通过液相色谱保留时间、峰纯度检查和光谱相似度检查对非法添加物进行确证。结果表明，该色谱条件下，7种抗寄生虫药物间分离度良好，在3～80 μg/mL浓度范围内线性关系良好（R2>0.9998），回收率在103.9%～105.8%之间，RSD≤2.5%，4种苯并咪唑类和3种大环内酯类的检测限分别为0.05 g/kg和0.3 g/kg。该方法操作简便、快速、灵敏度高，可用于硫酸新霉素可溶性粉中非法添加抗寄生虫药物的测定。 |
| 关键词: 硫酸新霉素可溶性粉 非法添加 抗寄生虫药物 高效液相色谱法 |
| DOI： |
| 投稿时间：2022-08-23修订日期：2023-04-03 |
| 基金项目:北京市科学技术协会2020-2022年度青年人才托举工程项目（No.118）；中国兽医药品监察所兽药行业公益性重点专项（No.GY202001、No.GY2021007） |
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| Determination of 4 Benzimidazoles and 3 Macrolides Antiparasitic Drugs Illegally Added in Neomycin Sulfate Soluble Powder by HPLC-PDA |
|
| (China Institute of Veterinary Drug Control) |
| Abstract: |
| A method was established for the determination of seven antiparasitic drugs in neomycin soluble powder by the high-performance liquid chromatography with photo-diode detector (HPLC-PDA). C18 column was applied with mobile phase consisting of acetonitrile and water, gradient elution, at a flow rate of 1.0 mL/min. The detection wavelength was 245 nm. Retention time, peak purity test and spectrum similar test were utilized to identify the antiparasitic drugs. seven antiparasitic drugs were detected through the established method, which showed a good limits of detection (0.05 g/kg of benzimidazoles and 0.3 g/kg of macrolides) and linear range (3～80 μg/mL) with a high correlation coefficients (r2>0.9998). The recoveries ranged from 103.9% to 105.8%. The established method is simple, rapid and sensitive, and can be utilized for the determination of antiparasitic drugs illegally added to neomycin sulfate soluble powder. |
| Key words: neomycin sulfate soluble powder illegally addition antiparasitic drugs high-performance liquid chromatography |
