| 摘要: |
| 目的 构建串联SpaA优势表位的丹毒亚单位疫苗，并对其免疫原性进行研究。方法 将丹毒杆菌表面保护抗原A优势表位串联表达，提取并经亲和层析纯化获得重组蛋白，制备亚单位疫苗，免疫小鼠和猪，21日后攻毒。结果 成功制备串联SpaA优势表位的丹毒亚单位疫苗，2μg重组蛋白可为小鼠抵抗1000MLD强毒攻击提供100%保护，100μg重组蛋白可为猪抵抗1MLD强毒攻击提供100%保护，可为猪抵抗2MLD强毒攻击提供67%保护。结论 串联SpaA优势表位的丹毒亚单位疫苗具有良好的免疫原性，高于商品化疫苗保护标准。 |
| 关键词: 串联 SpaA 优势表位 丹毒 疫苗 免疫原性 攻毒保护 猪 |
| DOI： |
| 投稿时间：2022-10-30修订日期：2023-10-23 |
| 基金项目:兽药行业公益性重点专项（GY202006） |
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| Construction of Erysipelas Subunit Vaccine with Tandem SpaA Dominant Epitopes |
|
| (China Institute of Veterinary Drug Control) |
| Abstract: |
| Abstract:Objective The purpose of this study was to construct a subunit vaccine of Erysipelas with the dominant epitopes of SpaA of Erysipelothrix erysipelas in tandem,and to study its immunogenicity.Methods The dominant epitopes of surface protection antigen A of Erysipelothrix erysipelas was expressed in tandem,and the recombinant protein was extracted and purified by affinity chromatography.The subunit vaccine was prepared and immunized mice and pigs which was challenged 21 days later.Results The Erysipelas subunit vaccine with the dominant epitopes of SpaA in tandem was successfully prepared.2μg recombinant protein can provide 100% protection for mice against 1000MLD virulent attack.100μg recombinant protein can provide 100% protection against the attack of 1MLD virulent attack and 67% protection against the attack of 2MLD virulent attack for pigs.Conclusion The Erysipelas subunit vaccine with the dominant epitope of SpaA in tandem has good immunogenicity, which is higher than the protection standard of commercial vaccine. |
| Key words: tandem SpaA dominant epitopes Erysipelas vaccine immunogenicity protection against challenge pig |
