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超高效液相色谱-串联质谱法测定猪牛组织中甲基盐霉素残留量
王丽娜,郭思琦,田晓玲,刘鸿鹤,郭国贤,兰雨濛,孙微微
0
(辽宁省检验检测认证中心)
摘要:
建立超高效液相色谱-串联质谱法测定猪牛组织中甲基盐霉素残留量。猪、牛组织中残留的甲基盐霉素,经乙腈溶液提取,硅胶固相萃取柱净化,超高效液相色谱-串联质谱法测定,外标法定量。甲基盐霉素在5 ng/mL~250 ng/mL的浓度范围内呈现良好的线性关系,甲基盐霉素在猪、牛肌肉、肝脏、肾脏、脂肪中的检测限均为2.5 μg/kg,定量限均为5.0 μg/kg,猪牛肌肉、肝脏、肾脏、脂肪中5.0 ng/g~100 ng/g添加浓度范围内的回收率均值为71.9%~88.4%,批内批间RSD值均<15%。
关键词:  猪牛组织  甲基盐霉素  超高效液相色谱-串联质谱法  
DOI:
投稿时间:2022-12-29修订日期:2023-04-18
基金项目:
Determination of Narasin Residues in the Tissue of Pig and Cattle by High Performance Liquid Chromatography-Tandem Mass Spectrometry
(Animal feed quality and safety of venterinary testing centre in Liaoning province Shenyang China)
Abstract:
A method of high performance liquid chromatography- tandem mass spectrometry for determination of narasin in the tissue of pig and cattle was developed. The samples were extracted with acetonitrile, and cleaned up with silica solid phase extraction cartridg.The separation of the target was carried out on a C18column by elution with methanol-0.2% formic acid as mobile phase, The results showed that correlation coefficient was 0.9999.The LOD and LOQ were 2.5 μg/kg and 5.0 μg/kg.The average recoveries were between 71.9%~88.4% at spiked levels of 5.0~100 μg/kg.The RSD were below 15%.The real sample tests showed this method can be used for the sensitive and accurate determination of narasin residues in the tissue of cattle and pig.
Key words:  the tissue of pig and cattle  narasin residues  UPLC-MS/MS

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