| 摘要: |
| 为了建立同时测定中兽药茵栀解毒颗粒中绿原酸、栀子苷和黄芩苷的方法，研究采用高效液相色谱法，选用C18色谱柱（250 mm×4.6 mm，5 μm），乙腈和0.05%磷酸溶液为流动相，流速1.0 mL/min，进样量10 μL，柱温为30 ℃，检测波长为230 nm。结果表明，栀子苷在3.110～155.5 μg/mL、黄芩苷在1.897～94.86 μg/mL、绿原酸在2.478～123.9 μg/mL浓度范围内线性关系良好，相关系数均大于0.999，平均加样回收率分别为97.1%、96.2%和95.3%。该试验操作简便、快捷、定性、定量准确，能满足中兽药茵栀解毒颗粒中3种有效成分同时检测的需要。 |
| 关键词: 茵栀解毒颗粒 绿原酸 栀子苷 黄芩苷 高效液相色谱法 |
| DOI： |
| 投稿时间：2023-03-20修订日期：2023-09-09 |
| 基金项目: |
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| Research on the Simultaneous Determination of Three Active Ingredients in Gardenia Jasminoides Granules by HPLC |
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| (Greentown Agricultural Testing Technology Co., Ltd) |
| Abstract: |
| In order to establish a method for the simultaneous determination of chlorogenic acid, gardenia glucoside and baicalin in the Chinese veterinary medicine gardenia antidotes, a C18 column (250 mm×4.6 mm, 5 μm) with acetonitrile and 0.05% phosphoric acid as the mobile phases was used, the flow rate was 1.0 mL/min, the injection volume was 10 μL, the column temperature was 30 ℃ and the detection wavelength was 230 nm. The linearity of the method was good in the range of 3.110 μg/mL to 155.5 μg/mL for gardenia glucoside, 1.897 μg/mL to 94.86 μg/mL for baicalin and 2.478 μg/mL to 123.9 μg/mL for chlorogenic acid, with the correlation coefficients greater than 0.999. The average recoveries were 97.1%, 96.2% and 95.3%, respectively. The test is simple, rapid, qualitative and quantitative, and can meet the needs of the simultaneous determination of the three active ingredients in the Chinese veterinary medicine gardenia antidote granules. |
| Key words: yinzhi jiedu granule chlorogenic acid gardenia glycosides baicalin high performance liquid chromatography |
