引用本文
  •    [点击复制]
  •    [点击复制]
【打印本页】 【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 441次   下载 462 本文二维码信息
码上扫一扫!
HPLC法同时测定茵栀解毒颗粒中3种有效成分的研究
钟寒辉,王彬,陈晓林,余功富,林仙军
0
(绿城农科检测技术有限公司)
摘要:
为了建立同时测定中兽药茵栀解毒颗粒中绿原酸、栀子苷和黄芩苷的方法,研究采用高效液相色谱法,选用C18色谱柱(250 mm×4.6 mm,5 μm),乙腈和0.05%磷酸溶液为流动相,流速1.0 mL/min,进样量10 μL,柱温为30 ℃,检测波长为230 nm。结果表明,栀子苷在3.110~155.5 μg/mL、黄芩苷在1.897~94.86 μg/mL、绿原酸在2.478~123.9 μg/mL浓度范围内线性关系良好,相关系数均大于0.999,平均加样回收率分别为97.1%、96.2%和95.3%。该试验操作简便、快捷、定性、定量准确,能满足中兽药茵栀解毒颗粒中3种有效成分同时检测的需要。
关键词:  茵栀解毒颗粒  绿原酸  栀子苷  黄芩苷  高效液相色谱法
DOI:
投稿时间:2023-03-20修订日期:2023-09-09
基金项目:
Research on the Simultaneous Determination of Three Active Ingredients in Gardenia Jasminoides Granules by HPLC
(Greentown Agricultural Testing Technology Co., Ltd)
Abstract:
In order to establish a method for the simultaneous determination of chlorogenic acid, gardenia glucoside and baicalin in the Chinese veterinary medicine gardenia antidotes, a C18 column (250 mm×4.6 mm, 5 μm) with acetonitrile and 0.05% phosphoric acid as the mobile phases was used, the flow rate was 1.0 mL/min, the injection volume was 10 μL, the column temperature was 30 ℃ and the detection wavelength was 230 nm. The linearity of the method was good in the range of 3.110 μg/mL to 155.5 μg/mL for gardenia glucoside, 1.897 μg/mL to 94.86 μg/mL for baicalin and 2.478 μg/mL to 123.9 μg/mL for chlorogenic acid, with the correlation coefficients greater than 0.999. The average recoveries were 97.1%, 96.2% and 95.3%, respectively. The test is simple, rapid, qualitative and quantitative, and can meet the needs of the simultaneous determination of the three active ingredients in the Chinese veterinary medicine gardenia antidote granules.
Key words:  yinzhi jiedu granule  chlorogenic acid  gardenia glycosides  baicalin  high performance liquid chromatography

用微信扫一扫

用微信扫一扫