引用本文
  •    [点击复制]
  •    [点击复制]
【打印本页】 【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 301次   下载 391 本文二维码信息
码上扫一扫!
兽用中药玉屏风口服液质量标准提升研究
冯亚文,张秋娜,吴兰,陈静,张娜,李会平,张培训,安莎莎,孔红军
0
(河北省兽药饲料工作总站)
摘要:
目的:建立一种可同时测定玉屏风口服液中升麻素苷、5-O-甲基维斯阿米醇苷、毛蕊异黄酮葡萄糖苷含量的高效液相色谱法(HPLC)。方法:采用以十八烷基硅烷键合硅胶为填充剂的色谱柱(5μm 4.6×250mm),以乙腈和0.2%磷酸溶液为流动相进行梯度洗脱,流速1.0mL·min-1,柱温30℃,检测波长254nm,进样体积10μL。结果:检测的三种成分在测定的质量浓度范围内线性关系良好,r值均为0.999,平均加样回收率均在96%~102%之间,相对标准偏差均小于2.0%。试验精密度、重复性、准确性和稳定性良好。结论:新建立的检测方法简单、准确、高效,为进一步提升玉屏风口服液的质量控制提供了依据。
关键词:  高效液相色谱  玉屏风口服液  升麻素苷  5-O-甲基维斯阿米醇苷  毛蕊异黄酮葡萄糖苷  
DOI:
投稿时间:2023-04-06修订日期:2023-12-13
基金项目:
Study on improvement of quality standard of Yupingfeng oral liquid
(Hebei provincial station of veterinary drug feed,shijiazhuang)
Abstract:
[Abstract]Objective:To establish a high performance liquid chromatography (HPLC) method for the simultaneous determination of glucoside, 5-O-methyl Visamitol side and calynoisoflavone glucoside in Yupingfeng oral liquid. Methods: The chromatography was performed on a column (5μm 4.6×250mm) with acetonitrile and 0.2% phosphoric acid as the fillers. The flow rate was 1.0mL·min-1. The column temperature was 30℃. The detection wavelength was 254nm and the sample volume was 10μg. Results: The linear relationship of the three components was good in the range of mass concentration, r values were 0.999, the average recoveries were between 96% and 102%, and the relative standard deviations were less than 2.0%. The test has good precision, repeatability, accuracy and stability. Conclusion: The newly established detection method is simple, accurate and efficient, which can provide a basis for the quality control of Yupingfeng oral liquid.
Key words:  HPLC  Yupingfeng oral liquid  Glucoside  5-O-methylvisamitol glycoside  Calycosin isoflavones glucoside.

用微信扫一扫

用微信扫一扫