| 摘要: |
| 为了探究西黄丸（XHP）在体内、体外抗乳腺肿瘤的作用，本实验采用CCK-8法测定XHP对犬乳腺肿瘤细胞系（CMT-U27）的细胞增殖抑制率；将荷瘤小鼠随机分为对照组（Control）、多柔比星组（DOX）、西黄丸低剂量组（XHP-L）、西黄丸中剂量组（XHP-M）和西黄丸高剂量组（XHP-H），考察不同剂量XHP对CMT-U27皮下移植瘤模型的抑瘤药效。体外试验结果显示，与对照组相比，XHP可显著抑制CMT-U27细胞的生长，并呈时间与浓度依赖性。动物试验结果显示，各试验组肿瘤体积显著小于对照组（P＜0.05），西黄丸组肿瘤重量显著下降。其中，西黄丸低、中、高剂量组的抑瘤率分别为15.3%、20.53%和57.99%。与DOX组裸鼠相比，西黄丸组裸鼠精神良好，体重增长及脏器系数正常。综上所述，西黄丸对犬乳腺肿瘤细胞系CMT-U27有显著增殖抑制作用，对裸鼠皮下移植瘤具有良好的抑瘤效果，且对机体无明显毒副作用，为治疗犬乳腺肿瘤提供新的选择。 |
| 关键词: 西黄丸 乳腺肿瘤 抗肿瘤 |
| DOI： |
| 投稿时间：2024-09-26修订日期：2024-12-01 |
| 基金项目: |
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| Study on the anti-tumour effect of Xihuang pill on canine mammary tumour cells and tumor of nude mice |
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| Abstract: |
| To investigate the anti-mammary tumor effects of Xihuang pill (XHP) in vivo and in vitro, the CCK-8 assay was used to determine the inhibition rate of XHP on the cell proliferation of canine mammary tumor cell line (CMT-U27); the tumor-bearing mice were randomly divided into Control, Doxorubicin, Xihuang pill Low Dose (XHP-L), Xihuang pill Medium Dose (XHP-M), and XHP-H group to investigate the tumor inhibitory efficacy of different doses of XHP on the CMT-U27 subcutaneous transplantation tumor model. ) and Xihuang pill high-dose group (XHP-H) to investigate the tumor-suppressive efficacy of different doses of XHP on the CMT-U27 subcutaneous graft tumor model. The results of in vitro tests showed that XHP significantly inhibited the growth of CMT-U27 cells in a time- and concentration-dependent manner compared with the control group. The results of animal tests showed that the tumor volume of each test group was significantly smaller than that of the control group (P?< 0.05), and the tumor weight of the XHP group was significantly decreased. Among them, the tumor inhibition rates of Xihuang pill low, medium and high dose groups were 15.3%, 20.53% and 57.99%, respectively. Compared with the nude mice in the DOX group, the nude mice in the Xihuang pill group were in good spirits, with normal weight gain and organ coefficients. In conclusion, the Xihuang pill has a significant proliferation inhibitory effect on canine mammary tumor cell line CMT-U27, has a good tumor suppression effect on subcutaneous transplantation tumor in nude mice, and has no apparent toxic side effects on the organism, providing a new choice for the treatment of canine mammary tumors. |
| Key words: Xihuang Pill mammary tumour anti-tumor |
