| 摘要: |
| 本实验建立了采用高效液相色谱技术（HPLC）测定大承气散中大黄酚、大黄酸、大黄素、芦荟大黄素、大黄素甲醚的含量。采用HypersilGOLD （250mm×4.6mm，5um），以0.1％磷酸溶液为流动相A、以乙腈为流动相B进行梯度洗脱，流速1.0mL/min，检测波长为225nm，柱温为40℃，进样体积为20ul。大黄酚在10.0～100.0μg范围内线性关系良好，平均回收率为101.4%（RSD=1.5%）；大黄酸在10.5～105.0μg范围内线性关系良好，平均回收率为98.1%（RSD=1.7%）；大黄素在11.0～110.0μg范围内线性关系良好，平均回收率为99.4%（RSD=2.1%）；芦荟大黄素在14.0～140.0μg范围内线性关系良好，平均回收率为99.8%（RSD=2.1%）；大黄素甲醚在15.7～157μg范围内线性关系良好，平均回收率为98.4%（RSD=1.7%）。结果表明该方法有较好的分离度、准确度，可用于测定大承气散中大黄酚、大黄酸、大黄素、芦荟大黄素、大黄素甲醚的含量，为大承气散的质量评价提供数据支持。 |
| 关键词: 大承气散 大黄酚 大黄酸 大黄素 芦荟大黄素 大黄素甲醚 |
| DOI： |
| 投稿时间：2024-11-09修订日期：2025-05-09 |
| 基金项目: |
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| (Enshi Polytechnic) |
| Abstract: |
| Abstract:This experiment established a high-performance liquid chromatography (HPLC) technique to determine the content of chrysophanol，rhein，emodin，aloe-emodin and physcion in Dachengqisan.The sample was separated on a Hypersil GOLD (250mm×4.6mm,5um)with acetonitrile-0.1% phosphoric acid in gradient elution.The flow rate was 1.0 mL/min,The detection wavelength was 225nm,and the column temperature was 40℃,the injection volume was 20ul. The linear relationship of chrysophanol within the range of 10.0 to 100.0ug is good, with an average recovery rate of 101.4% (RSD=1.5%); The linear range of rhein was 10.5 to 105.0ug, with an average recovery rate was 98.1% (RSD=1.7%); The linear range of emodin within was 11.0 to 110.0ug, with an average recovery rate was 99.4% (RSD=2.1%);The linear range of aloe-emodin was 14.0 to 140.0ug,with an average recovery rate was 99.8% (RSD=2.1%); The linear range of physcion was 15.7 to 157.0ug,with an average recovery rate was 98.4% (RSD=1.7%). The results showed that the method has good separation and accuracy, and can be used to determine the content of chrysophanol，rhein，emodin，aloe-emodin and physcion in Dachengqisan, and provide data support for the quality evaluation of Dachengqisan. |
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