| 摘要: |
| 利用超高效液相色谱-高分辨四极杆飞行时间质谱对预混剂中的黄霉素5种组分进行鉴别分析。采用C18色谱柱分离，以乙腈-0.1%甲酸水溶液(含2 mmol/L乙酸铵)为流动相梯度洗脱，流速0.4 mL/min，柱温40 ℃；质谱条件为电喷雾离子源，检测方式为负离子全扫描模式，通过保留时间、精确分子量和二级质谱特征碎片完成对黄霉素的5种组分的鉴别。与高效液相色谱方法比较，本方法具有判断准确、检测快速的特点，具有很好的应用价值。 |
| 关键词: 鉴别 黄霉素 超高效液相色谱 高分辨四极杆飞行时间质谱 |
| DOI： |
| 投稿时间：2016-02-24修订日期：2016-03-31 |
| 基金项目:国家高技术研究发展计划 (2011AA10A214) |
|
| Indentification of Flavomycin in Premix by Ultra-Performance Liquid Chromatography-Tandem High Resolution Quadrupole Time-of-Flight Mass Spectrometry |
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| (Key Laboratory of Biological Products and Chemical Drugs for Animals,Ministry of Agriculture,Beijing Engineering Research Center of Design and Development of Synthetic Peptide Vaccines for Animals,China Animal Husbandry Industry Co,Ltd) |
| Abstract: |
| Using ultra-performance liquid chromatography-tandem high resolution quadrupole time-of-flight mass spectrometry, the discriminant analysis of 5 components of flavomycin in premix was conducted. Based on C18 chromatogram column separation, the acetonitrile - 0.1% formic acid aqueous solution (including 2 mmol/L ammonium acetate) was employed as the mobile phase at flow rate of 0.4 mL/min for gradient elution at column temperature of 40 ℃; Mass spectrometry conditions were electrospray ionization (ESI) operated in negative ion full scan mode. The identification of 5 components of flavomycin were implemented based on retention time, exact molecular weight, and secondary mass spectral characteristic fragments. Compared with high performance liquid chromatography, this method is of better application value for its advantages of accurate judgement and rapid detection. |
| Key words: indentification flavomycin ultra-performance liquid chromatography high resolution quadrupole time-of-flight mass spectrometry |
