| 摘要: |
| 建立了兽用复方氨基比林注射液二组分含量测定的高效液相色谱法。以C18柱为固定相，0.05 mol/L的磷酸二氢钾（用三乙胺调节pH值到8.2）:乙腈=75: 25为流动相，流速为1.0 mL/min，柱温30℃，检测波长240 nm。采用外标法定量计算含量。巴比妥的线性范围为5.6~280μg/mL，回归方程为Area=21.856×Amt+42.143(n=5)，相关系数R2=1.0000；氨基比林的线性范围为14.3~715.0μg/mL，回归方程为Area = 22.267×Amt-13.009 (n=5)，相关系数R2= 1.0000。巴比妥80%、100%、120%三个添加水平的回收率在97.86%~101.06%之间，相应的氨基比林的回收率在99.48%~100.84%之间。方法专属性强，灵敏度佳，准确度高，能够有效控制兽用复方氨基比林注射液的质量。 |
| 关键词: 高效液相色谱法 复方氨基比林注射液 含量 |
| DOI： |
| 投稿时间：2017-03-02修订日期：2017-08-09 |
| 基金项目:无 |
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| Simultaneous Determination of Two Component in Veterinary Compound Aminophenazone Injection by HPLC |
|
| (Guizhou Provincial Supervisory Institute of Veterinary Drug and Feeds,Guiyang) |
| Abstract: |
| A method was developed to determine two component of veterinary Compound Aminophenazone Injection by HPLC, with C18 column as stationary phase, a solution of 0.05mol/L potassium dihydrogen?phosphate ( pH adjusted to 8.2 by Triethylamine )－acetonitrile = 75 : 25 as mobile phase，flow rate of 1.0 ml/min, column temperature of 30 degree Celsius, and wavelength of 240 nm. It was quantified by external standard method and the linear range of barbital was between 5.6 and 280.0 μg/mL, and the regression equation was Area = 21.856×Amt + 42.143, R2 =1.0000; the linear range of aminophenazone was between 14.3 and 715.0 μg/mL, and the regression equation was Area = 22.267×Amt-13.009, R2 =1.0000. Result showed that recoveries of 80%, 100%, 120% adding level of barbital were between 97.86% to 101.06%, and relative recoveries of aminophenazone were between 99.48% to 100.84%. The method was a precise, specific and accurate method, could be used for determining the content of veterinary compound aminophenazone injection. |
| Key words: HPLC compound aminophenazone injection content. |
