| 摘要: |
| 为了测定去滞散中枳壳的含量，采用C18柱（4.6×150mm，5μm），在室温下，以乙腈-水（20：80，用磷酸调节pH值至3）为流动相，283nm为检测波长，流速为1.0ml?min-1，建立了反相高效液相色谱（RP-HPLC）测定方法。结果显示，柚皮苷在0.08～4μg范围内线性关系良好，R2 = 0.999 9，回收率为94.4% ~101.7%，RSD为2.23%；新橙皮苷在0.08～4μg范围内线性关系良好，R2 = 0.999 5，回收率为90.6% ~100.0%，RSD为2.85%。本方法简便、准确、快速，可用于控制去滞散中枳壳的含量。 |
| 关键词: 去滞散 枳壳 柚皮苷 新橙皮苷 高效液相色谱法 |
| DOI： |
| 投稿时间：2017-06-04修订日期：2017-12-01 |
| 基金项目:公益性行业(农业)科研专项(项目编号：20130340-15) |
|
| RP-HPLC determination of Naringin and Neohesperidin in Quzhi San |
|
| (Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS,Lanzhou,;Gansu Institute of Veterinary Drug and Feed Inspection) |
| Abstract: |
| A method for the determination of Aurantii fructus in Quzhi San was developed by the reverse high performance liquid chromatography（RP-HPLC）.The C18 colum (4.6×150mm,5μm) was used with acetonitrle-water(20:80, pH was adjusted to 3 by H3PO4) and the detection wavelength was 283nm.The results showed that Naringin had a good linear relationship at the range of 0.08 ~ 4μg（R2 = 0.999 9）and the recovery rate were 94.4% ~101.7%，RSD=2.23%；Neohesperidin had a good linear relationship at the range of 0.08 ~ 4μg（R2 = 0.999 5）and the recovery rate were 90.6% ~100.0%，RSD=2.85 %. The method was simple, accurate and fast in the determination of Aurantii fructus in Quzhi San. |
| Key words: Quzhi San Aurantii fructus Naringin Neohesperidin RP-HPLC |
