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| 高效液相色谱法测定板蓝根颗粒中腺苷的含量 |
| 王亚芳,钟昆芮,李应超,周艳飞,张小飞,高婷,秦俊杰,王海军,战皓 |
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| (北京市兽药监察所;北京生泰尔生物科技有限公司) |
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| 摘要: |
| 建立了兽用板蓝根颗粒中腺苷含量测定的高效液相色谱法。以Symmetry C18柱为固定相,甲醇-0.05mol/L磷酸二氢钾溶液(10：90)为流动相,检测波长260nm，流速为1.2ml/min，柱温30℃。采用外标法定量计算含量。腺苷的线性范围为4～20μg/ml，回归方程y=71169x-6732，R2=0.9999；平均回收率96.62%，RSD为 1.08%。方法操作简单、精密度高、稳定性好，测定结果准确可靠，可用于板蓝根颗粒中腺苷的质量控制。 |
| 关键词: 腺苷 板蓝根颗粒 高效液相色谱法 质量控制 |
| DOI： |
| 投稿时间：2017-12-21修订日期：2018-07-03 |
| 基金项目: |
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| Determination of Adenosine in Banlangen Granules by HPLC |
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| (Beijing Institute of Veterinary Drug Control) |
| Abstract: |
| A method was developed to determine the content of Adenosine in Banlangen Granules by HPLC, with Symmetry C18 column as stationary phase, mobile phase was methyl alcohol-0.05 mol/L potassium dihydrogen phosphate (10:90),detection wavelength was 260 nm, flow rate was 1.2 mL/min, and column temperature was 30 ℃. It was quantified by external standard method and adenosine in sample quantity is 4~20 μg/ml range, show a good linear relationship, y=71169x-6732(R2=0.9999). The recovery was 96.62%, RSD was 1.08%. This method is simple and accurate with good stability, it is suitable for determining the content of Adenosine in Banlangen Granules. |
| Key words: Adenosine Banlangen Granules HPLC quality control |
